Lipid composition of integral purple membrane by 1H and 31P NMR
نویسندگان
چکیده
منابع مشابه
Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane.
The lipid/protein stoichiometries of a naturally crystalline biological membrane, the purple membrane (PM) of Halobacterium salinarum, have been obtained by a combination of (31)P- and (1)H-NMR analyses of the lipid extract. In total, 10 lipid molecules per retinal were found to be present in the PM lipid extract: 2-3 molecules of phosphatidylglycerophosphate methyl ester (PGP-Me), 3 of glycoli...
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Proton, deuterium and carbon magnetic resonance techniques have been widely used to study the hydrocarbon chain region of model bilayer systems and biological membranes (for a review see :[l]). More recently, phosphorus magnetic resonance (31P NMR) has been introduced to study the polar headgroup region of membranes [2-7]. In this communication we show how 31P NMR can define an order parameter ...
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Various motionally averaged 31P-1H, 13C-1H, 1H-1H, and 31P-13C dipolar couplings were measured for natural-abundance and unoriented phosphocholine in the L alpha phase. The couplings were obtained and assigned by a variety of advanced and partly novel two-dimensional solid-state NMR experiments. Whereas 31P-1H and 31P-13C dipolar couplings provide long-range structural constraints, geminal 1H-1...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2005
ISSN: 0022-2275
DOI: 10.1194/jlr.m500138-jlr200